The Aquachrom LLC Scientific Research Centre provides services for solving a wide range of analytical problems for the biopharmaceutical and food industries.
The main activity of the laboratory is the study of the structure of therapeutic proteins (including monoclonal antibodies and peptides), primarily by high-resolution mass spectrometry methods.
In particular, the Centre can solve the following tasks:
- confirmation of the amino acid sequence of the protein (including the N- and C-terminal sections);
- peptide mapping;
- analysis of amino acid substitutions;
- analysis of post-translational modifications (oxidation, deamidation, and many others);
- analysis of disulfide bonds (position of disulfide bridges, state of cysteine residues, recombination);
- analysis of glycosylated proteins – determination of the state of glycosylation sites, glycan structure, construction of a glycosylation profile (“glycan profile”), analysis of glycopeptides, analysis of released glycans;
- biosimilarity research;
- assessment of protein stress stability, including identification of products formed under conditions of accelerated degradation;
- studies of the secondary and tertiary structure of the protein by auxiliary methods: analysis of circular dichroism and fluorescence spectra;
- development and validation of analytical methods
The laboratory centre is provided with modern laboratory equipment: Waters SYNAPT XS HDMS (QTof-IMS) high-resolution mass spectrometer with the Waters ACQUITY Premier chromatograph (HPLC).
For the food and perfumery and cosmetics industries, the centre can determine the fatty acid composition of products, the chromatographic profile of essential oils using gas chromatography with mass detection and other studies.
Contacts: nic@aquachrom.ru